Thomas Laue, Ph.D.

Tom Laue

 

 Professor

 Director, Biomolecular Interaction Technologies Center (BITC)
 Director, Center to Advance Molecular Interaction Science
  (CAMIS)

 Rudman Hall, Room 379
 (603) 862-2459   
 Email: Tom.Laue@unh.edu

 

 

Educational Background:

Ph.D.,  University of Connecticut. Area: Biophysics and Biochemistry, 1981
B.A.. The Johns Hopkins University. Area: Natural Sciences, 1971 

Research Interests:

The research in my laboratory, the Center to Advance Molecular Interaction Science (CAMIS), is directed toward the development of direct physical methods for determining properties of macromolecules, including mass, charge, size, shape, and solubility, as well as the state of aggregation and the extent of ligand binding. Along with standard optical methods (absorbance, fluorescence, circular dichroism), our lab has developed novel instrumentation for sedimentation analysis (both equilibrium and velocity) and charge analysis (steady state electrophoresis). These methods are being applied to a wide range of macromolecular interactions of importance, including those in blood coagulation, DNA transcriptional regulation, viral DNA integration, and gel formation. Moreover, we are now studying the contribution of macromolecular charge to structure, stability, and assembly through a unique combination of physical methods.

Industry/University Cooperative Research Center:

The Biomolecular Interaction Technologies Center (BITC) is a National Science Foundation-sponsored consortium of academic institutions and industrial partners. BITC member companies underwrite research projects and the development of resources in cutting-edge technologies, particularly those of interest in the development of safe and effective drugs. Member companies include pharmaceutical firms and instrument manufacturers. BITC holds annual workshops at UNH on methods and technologies essential to the study of biomolecular interactions, and opens the workshop lectures to UNH scientists and students.

Professional Positions:

Director, Biomolecular Interaction Technologies Center, 2000
Director, Center to Advance Molecular Interaction Science, 1999
Professor, Biochemistry and Molecular Biology, U. New Hampshire, 1995
Associate Professor, Biochemistry and Molecular Biology, U. New Hampshire, 1989
Assistant Professor, Biochemistry and Molecular Biology, U. New Hampshire, 1989
Research Associate, Chemistry, U. Oklahoma, 1981

Awards and Professional Activities

Named University of New Hampshire Outstanding Associate Professor - 1994
Genentech Research Prize ($10,000) - 1995
Editorial board member, Journal of Biological Chemistry - 2005
Scientific and Technical Advisory Board member, BioRad, Inc. - 2005
Keynote spearker - Gordon Conference RASMB - January 2006
Co-founder, Spin Analytical, Inc. - 2006
Keynote speaker - Institute for International Research - March 2006
University of New Hampshire Excellence in Research Award - 2004
Named Carpenter Professor, University of New Hampshire - 2008

Honors:

University of New Hampshire Outstanding Associate Professor, 1994
Genentech Research Prize, 1995

Selected Publications:

Books:
Schuster, T.M. and Laue, T.M. (eds.) "Modern Analytical Ultracentrifugation: Acquisition and Interpretation of Data for Biological and Synthetic Polymer Systems" (1994),  351 pp., 118 figures, Birkhäuser, Boston, MA.

Bonner, R.F., Cohn, G.E., Laue, T.M. and Priezzhev, A.V. (eds.) "Biochemical Diagnostic Instrumentation" (1994), SPIE Proceedings, Vol. 2136, 354 pp., SPIE, Bellingham, WA.

Chapters:

 Laue, T.M. (2010) “Sedimentation". In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/
9780470015902.a0002982.pub2]

Laue, T.M. (2010) "Applications of Sedimentation Velocity Analytical Ultracentrifugation." in Formulation and Process Development Strategies for Manufacturing Biopharmaceuticals, F. Jameel and S. Hershenson, eds. J. Wiley, New York, N.Y.

Cole, J.L., Lary, J.W., Moody, T.P., and Laue, T.M. (2007) "Analytical Ultracentrifuation: Sedimentation Velocity and Sedimentation Equilibrium." Methods in Cell Biology, Volume 84, Chapter, 6, pp. 143-179, J.J. Correia and W. Detrich, L. Han., eds., Elsevier, Holland.

Laue, T.M.
(2004) "Analytical Ultracentrifugation: A Powerful "New" Technology" in Drug Discovery Today: Technologies, 1:309-315.

Laue, T.M. (1999) "Analytical Sedimentation: Equilibrium Approach" in Current Protocols in Protein Science, Paul Wingfield, ed. 12 pp. Wiley-Interscience, New York.

Laue, T.M. and Stafford, W.F. III (1999) "Modern Applications of Analytical Ultracentrifugation" in Annual Review of Biophysics and Biomolecular Structure Vol. 28, 75-100, Ann. Rev.

Senear, D.F., Ross, J.B.A., and Laue, T.M. (1998) "Analysis of Protein and DNA Mediated Contributions to Cooperative Assembly of Protein-DNA Complexes" in Methoids: A Comparison to Methods in Enzymology. 16, 3-20, Academic Press, Inc. New York.

Laue, T.M., Shepard, H.K., Ridgeway, T.M., Moody, T.P., and Wilson T.J. (1998) "Membrane-Confined Analytical Electrophoresis" in Methods in Enzymology, Vol. 295, Ch. 24, pp. 479-490, G.K. Ackers and J.L. Johnson, eds., Academic Press, Inc. New York.

Laue, T.M., Anderson, A.L. and Weber, B.W. (1997) "Prototype Fluorescence Detector for the LA Analytical Ultracentrifuge" in Ultrasensitive Clinical Laboratory Diagnostics, SPIE Proceedings, Vol. 2985, pp. 196-204, G. Cohn and S. Soper eds., SPIE, Belllingham, WA.

Wu, Z., Johnson, K.W., Goldstein, B., Laue, T.M. and Ciardelli, T.L. (1996) "Solution Assembly of Cytokine Receptor Ectodomain Complexes" in Techniques in Protein Chemistry, Vol. VII, pp. 45-56, Academic Press, Inc., Boca Raton, FL.

Laue, T.M. (1996) "Analytical Ultracentrifugation" in Current Protocols in Protein Science, Paul Wingfield, ed., 12 pp. Wiley-Interscience, New York.

Laue, T.M. (1995) "Sedimentation Equilibrium as a Thermodynamic Tool" in Methods in Enzymology, Vol. 259, Ch. 19, pp. 427-452, G.K. Ackers and J.L. Johnson, editors, Academic Press, Inc. New York.

Yphantis, D.A., Lary, J.W., Stafford, W.F. III, Liu, S., Olsen, P.H., Hayes, D.B., Moody, T.P., Ridgeway, T.M., Lyons, D.A. and Laue, T.M. (1994) "On Line Data Acquisition for the Rayleigh Interference Optical System of the Analytical Ultracentrifuge" in Modern Analytical Ultracentrifugation: Acquisition and Interpretation of Data for Biological and Synthetic Polymer Systems., pp. 209-226, Schuster, T.M. and Laue, T.M. (eds.), Birkhauser, Boston, MA.

Articles:

Chahla, M., Wooll, J., Laue, T.M., Nguyen, N., and Senear, D.F. (2003) "Role of Protein-Protein Bridging Interactions on Cooperative Assembly of DNA-Bound CRP-CytR-CRP Complex and Regulation of the Escherichia Coli CytR Regulon". Biochemistry, 42, 3812-3825.

MacGregor, I.K., Anderson, A.L., and Laue, T.M. (2004) "Fluorescence Detection for the XLI Ultracentrifuge" Biophys. Chem., 108, 165-185.

Jiang, S., Jacobs, A., Laue, T.M., and Caffrey, M. (2004) "Solution structure of the coxsackievirus and adenovirus receptor doman 1." Biochemistry, 43, 1847-53.

Bou-Abdallah, F., Adinolfi, S., Pastore, A., Laue T.M., and Chasteen, N.D. (2004) "Iron binding and oxidation kinetics in frataxin CyaY a Escherichia coli." J Mol Biol. 341, 605-15.

Moody, T.P., Kingsbury, J.S., Durant, J.A., Wilson, T.J., Chase, S.F., and Laue, T.M. (2005) "Valence and anion binding of bovine ribonuclease A between pH 6 and 8." Anal. Biochem. 336, 243-252.

Laue, T.M., Austin, J.B., and Rau, D.A. (2006) "A Light Intensity Measurement System for the Analytical Ultracentrifuge." Progr Colloid Polym Sci., 131, 1-8.

Liu, Y., Chen, W., Gaudet, J., Cheney, M.D., Roudaia, L., Cierpicki, T., Klet, R.C., Hartman, K., Laue, T.M., Speck, N.A., and Bushweller, J.H. (2007) "Structural Basis for Recognition of SMRT/N-CoR by the MYND Doman and Its Contribution to AML1/ETO's Activity." Cancer Cell, 11, 483-497.

Yao, G., Chiang, Y-C., Zhang, C., Lee, D.J., Laue, T.M., and Denis, C.L. (2007) "PAB1 Self-association Precludes its Binding to Poly(A), Therby Accelerating CCR4 Deadenylation In Vitro." Mol. Cell. Biology, 27, 6243-6253.

Kingsbury, J.S., Klimtchuk, E.S., Laue, T.M., Theberge, R., Costello, C.E., and Connors, L.H. (in press) "The Modulation Of Transthyretin Tetramer Stability By Cysteine-10 Adducts and The Drug Diflunisal: Direct Analysis By Fluorescence-detected Analytical Ultracentrifugation." J. Biol. Chem.

Durant ,J.A., Chen, C., Laue, T.M., Moody, T.P., and Allison, S.A. (2002) "Use of T4 Lysozyme Charge Mutants to Examine Electrophoretic Models" Biophys. Chem., 101-102, 593-609.

Lukasik, S.M., Zhang, L.N., Corpora, T., Tomanicek, S., Li, Y.H., Kundu, M., Hartman, K., Liu, P.P., Laue, T.M., Biltonen, R.L., Speck, N.A., and Bushweller, J.H., (2002) "Altered affinity of CBF beta-SMMHC for Runx 1 explains its role in leukemogenesis" Nature Structural Biology, 9, 674-679.

Zhao, G., Ceci, P., Ilan, A., Giangiacomo, L., Laue, T.M., Chiancone, E., and Chasteen, N.D. (2002) "Iron and Hydrogen Peroxide Detoxification Properties of Dps: A Ferritin-like DNA Binding Protein of Escherichia coli." J. Biol. Chem. 277(31), 27689-27696.

Grady, J.K., Zang, J., Laue, T.M., Arosio, P. and Chasteen, N. D. (2002) “Characterization of the H and L Subunit Ratios of Ferritins by SDS Capillary Gel Electrophoresis” 302, 263-268.

Liparoto, S.F., Myszka, D.G., Wu, Z. Goldstein, B., Laue, T.M. , and Ciardelli, T.L., (2002) “Analysis of the Role of the Interleukin-2 Receptor γ Chain in Ligand Binding” Biochemistry, 41, 2543-2551.

Arcidiacono, S., Mello, C., Butler, M., Welsh, E., Soares, J., Allen, A., Ziegler, D., Laue, T.M. and Chase, S. (2002) “Aqueous Processing and Fiber Spinning of Recombinant Spider Silks” Macromolecules, 35, 1262-1266.

Hattan S., Laue, T.M. and Chasteen, D.C., (2001) “Purification and characterization of a novel calcium binding protein from the extrapallial  fluid of mollusc Mytilus edulis” J. Biol. Chem., 276, 4461-4468.

Kar, S.R., Kingsbury, J.S., Lewis, M.S., Laue, T.M. and Schuck, P. (2000) “Analysis of Transport Experiments using Pseudo-absorbance Data” Anal. Biochem. 285:135-142.

Tang, Y.-Y., Crute, B.E., Kelley, J.J., Huang, X., Yan, J., Shi, J., Hartman, K.L., Laue, T.M., Speck, N.A., and Bushweller, J.H. (2000) “Biophysical Characterization of Interactions between the Core Binding Factor α and β Subunits and DNA” FEBS Letters, 470, 167-172.

Coleman, J.L., Skalka, A.M., Merkel, G.W., Eaton, S.F. and Laue, T.M. (1999) "Characterization of the Self Association of Avian Sarcoma Virus Integrase by Analytical Ultracentrifugation" J. Biol. Chem., 274, 32842-32846.

Moncrieffe, M.C., Eaton S.F., Haydock, C., Potter, J.D., Laue T.M., and Prendergast F.G. (1999) Rotational and Translational Motion of Troponin C  J. Biol. Chem. 274: 17464-17470.

Allison, S.A., Wang, H., Laue, T.M., Wilson, T.J., and Wooll, J.O. (1999) “Visualizing Ion Relaxation in the Transport of Short DNA Fragments.” Biophys. J., 76, 2488-2501.

Wu, Z., Goldstein, B., Laue, T.M., Liparoto, S.E., Nemeth, M.J. and Ciardelli, T.L. (1999) “Solution Assembly of the Pseudo-high Affinity and Intermediate Affinity Interleukin-2 Receptor Complexes” Protein Science,  8, 482-489.

Ridgeway, T.M., Hayes, D.B., Moody, T.P., Wilson, T.J., Anderson, A.L., Levasseur, J.H., Demaine, P.D., Kenty, B.E., and Laue, T.M. (1998) "An Apparatus for Membrane-confined Analytical Electrophoresis." Electrophoresis, 19, 1611-1619.

Huang, X.M., Crute, B.E., Sun, C.H., Tang, Y.Y., Kelley, J.J., Lewis, A.F., Hartman, K.L., Laue, T.M., Speck, N.A. and Bushweller, J.H. (1998) “Overexpression, Purification and Biophysical Characterization of the Heterdimerization Domain of the Core-binding Factor-β Subunit.” J. Biol. Chem., 273, 2480-2487.

Senear, D.F., Rusinova, E., Ross, J.B.A., Laue, T.M. and Sowers, L.C. (1997) “Linkage between Operator Binding and Dimer to Octamer Self-Assembly of Bacteriophage λ cI Repressor” Biochemistry,  36, 12994-13003.

Revett, S.P., King, G., Shabanowitz, J., Hunt, D.F., Hartman, K.L., Laue, T.M. and Nelson, D.J. (1997) “Characterization of a Helix-Loop-Helix (EF Hand) Motif of Silver Hake Parvalbumin Isoform B” Protein Science, 6, 2397-2408.

Mehta, P., Patel, K.D., Laue, T.M., Erickson, H.P. and McEver, R.P. (1997) “Soluble Monomeric P-selectin Containing only the Lectin and EGF Domains Binds to PSGL-1 on Leukocytes” Blood, 90, 2381-2389.

Weissenhorn, W., Calder, L.J., Dessen, A., Laue, T., Skehel, J.J. and Wiley, D.C (1997) “Assembly and Crystallization of a Rod-Shaped Chimera of a Trimeric GCN4 Zipper and the HIV-1 Gp41 Ectodomain Expressed in E. coli” Proc. Natl. Acad. Sci., 94, 6065-6096.

Shepard, H.K., Wilson, T.J., Moody, T.P., Wooll, J.O., and Laue, T.M. (1997) “Determination of Macroion Diffusion Coefficients using an Analytical  Electrophoresis Apparatus” Eur. Biophys. J., 25, 481-487.

Patents:

Laue, T.M., Yphantis, D.A., and Hazard, A.L. "Apparatus and Method for Macromolecular Charge Determination" Pat. No. 4,994,161, granted 19 February 1991.

Technical Reports:

Laue, T.M. "Optical Systems of the XLA Ultracentrifuge" (1996) Applications Data Note for Spinco Division of Beckman Instruments, Inc. P.O.Box 10200, Palo Alto, California.

Laue, T.M. (1992) “Short Column Sedimentation Equilibrium Analysis for Rapid Characterization of Macromolecules in Solution.” Palo Alto, CA:Beckman Instruments, Inc., Technical Information DS835.

Laue, T.M., Schuster, T.M., Yphantis, D.A., Lewis, M.L. and Van Holde, K.H. (1986) "The Future of Analytical Ultracentrifugation" National Science Foundation, Biological Instrumentation Program report, 18 pp.

Green, R.H., Godfrey, J.F., Laue, E.G., Laue, T.M., Paik, W.W., and Wardle, M.D. (1972) "Quartz Crystal Microbalance Used in Biological Studies" NASA Tech Brief No. B72-10243.

Computer Programs:

Laue, T.M. (in preparation) “Chaos: Multi-component interaction simulator using chaos theory” C++ for Windows.

Laue, T.M. (in preparation) “XLIFdb: File database management for the XLI Analytical Ultracentrifuge” C++ and Access, written for Windows NT.

Laue, T.M. (in preparation) “XLI32 Operating System for the XLA Analytical Ultracentrifuge Version 1.x” 74000 lines of C++ code, written for Windows NT, multithreaded, OLE2 compatible container/sever, includes fluorescence/luminescence detection.

Laue, T.M. and Moody, T.P. (1995) “SseSim: Finite Difference Simulation of Broad-zone Analytical Electrophoresis” 21000 lines of C++ code, written for Windows 3.x, Windows 95 and a generic version (in C) for other C compilers.

Hayes, D.B., Philo, J.P. and Laue, T.M. (1994) “SedNTerp: Interpretation of Sedimentation Data Version 1.x” 2000 lines of Visual Basic code, written for Windows 3.x.

Laue, T.M. (1993) "XLA2000+ Operating System for the XLA Analytical Ultracentrifuge Version 2.x" 58000 lines of C++ code, written for Windows 3.1. (Commercialized version used by Beckman Instruments).

Laue, T.M. (1993) "XLA2000+ Operating System for the XLA Analytical Ultracentrifuge Version 1.x" 26000 lines of C++ code, written for Windows 3.1 (Prototype Version used only in lab)